This study was aimed to evaluate the effect of pomegranate peel extract on serum glucose, creatinine, serum\nuric acid and serum total protein in normal and Staphylococcus aureus infected mice. Thirty seven milk samples were\ncollected from Al-Kut city from cows suffering acute mastitis. After biochemical tests and API Staph System it was\nrevealed that 19 out of 37 milk samples contained S. aureus. Ethanolic extract of pomegranate peels was prepared and\nthe highest in vitro antibacterial effect was observed by 300mg/mL against S. aureus with 30.00 �± 0.57 mm of the\ndiameter zone. Thirty lactating mice were divided into three groups. Group (1): negative control (Normal). Group (2):\npositive control, mice were infected by intramammary injection of S. aureus at 7, 15, 21 and 30 days and left untreated.\nGroup (3): mice infected with intramammary injection of S. aureus and treated orally with 300 mg/kg BW of ethanolic\nextract of pomegranate peel after 48 h of infection for 15, 21 and 30 days. At the end of experiment, blood samples\nwere taken from anaesthetized mice then the mice were killed for histopathological examination and measured the\nfollowing: serum glucose, creatinine, serum uric acid and serum total proteins. Results showed significant increase (P\n<0.05) in the levels of serum glucose, creatinine, serum uric acid and serum total proteins in positive control mice as\ncompared with negative control while pomegranate peel treated group showed a significant (P <0.05) decrease in the\nlevels of serum glucose, creatinine, serum uric acid and serum total proteins. Histological section in mammary gland\nshowed clear pathological effects of S. aureus as compared with those treated with pomegranate peel extracts.
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